The application of cryogenic technology to medical problems has resulted in the development of methods for freeze-preserving two of the cellular components of blood. Cryopreservation of different cell types, e.g., nucleated leukocytes and non-nucleated erythrocytes, cannot be achieved successfully unless techniques designed to prevent cellular damage induced by freezing are used. Unprotected cells can, depending upon the rate of freezing, be damaged in a variety of ways, principally through dehydration, solute effects, and ice-crystal formation. When cryoprotective compounds, such as glycerol, DMSO, etc. are used and the cooling rate is controlled, cell damage can be circumvented, however. A slow cooling rate with an intracellular additive has been found better for preserving nucleated white blood cells, whereas very rapid freezing techniques have in general proven more successful for preserving non-nucleated red blood cells for transfusion. Ultra-rapid freezing of blood in the form of droplets is a useful means of preserving red cells for blood-group studies. Storage and prolonged preservation of biological specimens have been more successful at cryogenic temperatures (e.g., −196 deg C) than at higher storage temperatures (e.g., −85 deg C).
Skip Nav Destination
Preservation of Blood Components at Cryogenic Temperatures
Arthur W. Rowe
New York Blood Center, New York, N. Y.
- Views Icon Views
- Share Icon Share
- Search Site
Rowe, A. W. (May 1, 1972). "Preservation of Blood Components at Cryogenic Temperatures." ASME. J. Heat Transfer. May 1972; 94(2): 132. https://doi.org/10.1115/1.3449882
Download citation file:
Get Email Alerts
H1 and H2 Forced Convection in Semi-Elliptic Ducts
J. Heat Transfer