A type of persistent direction-changing positional nystagmus with a null point during head position deflection is known as light cupula syndrome (LCS) in the clinic. To date, the pathogenesis and biomechanical response of human semicircular canals with light cupula syndrome (LCS) (HSCs–LCS) are still unclear. In this study, based on the anatomical structure and size of the one-dimensional human semicircular canal (HSC) and imitating the pathological changes of the endolymph in HSC with LCS, a visual bionic semicircular canal (BSC) with LCS was fabricated using three-dimensional printing technology, hydrogel modification, and target tracking technology. Through theoretical derivation, mathematical models of the HSC–LCS perception process were established. By conducting in vitro experiments on the bionic model, the biomechanical response process of HSC–LCS was studied, and the mathematical models were validated. The results of pulse acceleration stimulation showed that the pathological changes in the density and viscosity of the endolymph could reduce the deformation of the cupula of the BSC–LCS and increase the time constant. The results of the sinusoidal acceleration stimulation showed that the amplitude–frequency gain of the BSC–LCS decreased and the phase difference increased. The BSC-LCS can be used as a tool for pathological research of the HSC–LCS. The results of this study can provide a theoretical basis for clinical diagnosis.