A combined experimental-numerical approach was adopted to characterize glucose and oxygen uptake and lactate production by bovine articular chondrocytes in a model system. For a wide range of cell concentrations, cells in agarose were supplemented with either low or high glucose medium. During an initial culture phase of , oxygen was monitored noninvasively using a biosensor system. Glucose and lactate were determined by medium sampling. In order to quantify glucose and oxygen uptake, a finite element approach was adopted to describe diffusion and uptake in the experimental model. Numerical predictions of lactate, based on simple relations for cell metabolism, were found to agree well for low glucose, but not for high glucose medium. Oxygen did not play a role in either case. Given the close association between chondrocyte energy metabolism and matrix synthesis, a quantifiable prediction of utilization can present a valuable contribution in the optimization of tissue engineering conditions.
Skip Nav Destination
Article navigation
October 2005
Technical Papers
Nutrient Utilization by Bovine Articular Chondrocytes: A Combined Experimental and Theoretical Approach
Bram G. Sengers,
Bram G. Sengers
Eindhoven University of Technology
, Department of Biomedical Engineering, P.O. Box 513, 5600 MB Eindhoven, The Netherlands
Search for other works by this author on:
Hannah K. Heywood,
Hannah K. Heywood
Queen Mary University of London
, Medical Engineering and IRC Biomedical Materials, Department of Engineering, Mile End Road, London E1 4NS, United Kingdom
Search for other works by this author on:
David A. Lee,
David A. Lee
Queen Mary University of London
, Medical Engineering and IRC Biomedical Materials, Department of Engineering, Mile End Road, London E1 4NS, United Kingdom
Search for other works by this author on:
Cees W. J. Oomens,
Cees W. J. Oomens
Eindhoven University of Technology
, Department of Biomedical Engineering, P.O. Box 513, 5600 MB Eindhoven, The Netherlands
Search for other works by this author on:
Dan L. Bader
Dan L. Bader
Eindhoven University of Technology
, Department of Biomedical Engineering, P.O. Box 513, 5600 MB Eindhoven, The Netherlands and Queen Mary University of London
, Medical Engineering and IRC Biomedical Materials, Department of Engineering, Mile End Road, London E1 4NS, United Kingdom
Search for other works by this author on:
Bram G. Sengers
Eindhoven University of Technology
, Department of Biomedical Engineering, P.O. Box 513, 5600 MB Eindhoven, The Netherlands
Hannah K. Heywood
Queen Mary University of London
, Medical Engineering and IRC Biomedical Materials, Department of Engineering, Mile End Road, London E1 4NS, United Kingdom
David A. Lee
Queen Mary University of London
, Medical Engineering and IRC Biomedical Materials, Department of Engineering, Mile End Road, London E1 4NS, United Kingdom
Cees W. J. Oomens
Eindhoven University of Technology
, Department of Biomedical Engineering, P.O. Box 513, 5600 MB Eindhoven, The Netherlands
Dan L. Bader
Eindhoven University of Technology
, Department of Biomedical Engineering, P.O. Box 513, 5600 MB Eindhoven, The Netherlands and Queen Mary University of London
, Medical Engineering and IRC Biomedical Materials, Department of Engineering, Mile End Road, London E1 4NS, United KingdomJ Biomech Eng. Oct 2005, 127(5): 758-766 (9 pages)
Published Online: May 26, 2005
Article history
Received:
December 22, 2004
Revised:
May 26, 2005
Citation
Sengers, B. G., Heywood, H. K., Lee, D. A., Oomens, C. W. J., and Bader, D. L. (May 26, 2005). "Nutrient Utilization by Bovine Articular Chondrocytes: A Combined Experimental and Theoretical Approach." ASME. J Biomech Eng. October 2005; 127(5): 758–766. https://doi.org/10.1115/1.1993664
Download citation file:
Get Email Alerts
Related Articles
Parametric Finite Element Analysis of Physical Stimuli Resulting From Mechanical Stimulation of Tissue Engineered Cartilage
J Biomech Eng (June,2009)
A Finite Element Model of Cell-Matrix Interactions to Study the Differential Effect of Scaffold Composition on Chondrogenic Response to Mechanical Stimulation
J Biomech Eng (April,2011)
An Integrated Finite-Element Approach to Mechanics, Transport and Biosynthesis in Tissue Engineering
J Biomech Eng (February,2004)
Principles of Tissue Engineering With Nonthermal Irreversible Electroporation
J. Heat Transfer (January,2011)
Related Proceedings Papers
Related Chapters
Summary and future direction
Nanomaterials in Glucose Sensing: Biomedical & Nanomedical Technologies - Concise Monographs
Synthesis and Characterization of Carboxymethyl Chitosan Based Hybrid Biopolymer Scaffold
International Conference on Mechanical and Electrical Technology, 3rd, (ICMET-China 2011), Volumes 1–3
A European View on Risk Management Strategies for Tissue Engineered Medical Products (TEMPs)
Tissue Engineered Medical Products (TEMPs)