Delivery of poorly water-soluble drug molecules, which constitute a large part of commercially available drugs, is a major challenge. Several drugs including paclitaxel (PTX) that are used for cancer treatment are hydrophobic and need to be delivered using an appropriate carrier. Here we engineered PTX-loaded polyelectrolyte films and microcapsules by pre-complexing PTX with alkylamino hydrazide derivatives of HA, and subsequent assembly with either poly(L-lysine) (PLL) or quaternized chitosan (QCHI) as polycations. The PTX loading capacity of the films was found to be dependent on the number of layers in the films as well as on the initial concentration of PTX pre-complexed to hydrophobic HA, with a loading capacity up to 5000-fold the initial PTX concentration. The films were stable in physiological medium and were degraded in the presence of hyaluronidase. The PTX-loaded microcapsules were found to decrease the viability and proliferation of MDA-MB-231 breast cancer cells, while unloaded microcapsules did not impact cell viability. All together, our results highlight the potential of hyaluronan-based assemblies for hydrophobic drug delivery.
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ASME 2012 Summer Bioengineering Conference
June 20–23, 2012
Fajardo, Puerto Rico, USA
Conference Sponsors:
- Bioengineering Division
ISBN:
978-0-7918-4480-9
PROCEEDINGS PAPER
Polyelectrolyte Multilayer Nanoshells With Hydrophobic Nanodomains for Delivery of Paclitaxel
Thomas Boudou,
Thomas Boudou
Grenoble Institute of Technology and CNRS, Grenoble, France
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Prathamesh Kharkar,
Prathamesh Kharkar
Grenoble Institute of Technology and CNRS, Grenoble, France
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Jing Jing,
Jing Jing
Centre de Recherches sur les Macromolécules Végétales, Grenoble, France
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Raphael Guillot,
Raphael Guillot
Grenoble Institute of Technology and CNRS, Grenoble, France
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Isabelle Pignot-Paintrand,
Isabelle Pignot-Paintrand
Centre de Recherches sur les Macromolécules Végétales, Grenoble, France
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Rachel Auzely-Velty,
Rachel Auzely-Velty
Centre de Recherches sur les Macromolécules Végétales, Grenoble, France
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Catherine Picart
Catherine Picart
Grenoble Institute of Technology and CNRS, Grenoble, France
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Thomas Boudou
Grenoble Institute of Technology and CNRS, Grenoble, France
Prathamesh Kharkar
Grenoble Institute of Technology and CNRS, Grenoble, France
Jing Jing
Centre de Recherches sur les Macromolécules Végétales, Grenoble, France
Raphael Guillot
Grenoble Institute of Technology and CNRS, Grenoble, France
Isabelle Pignot-Paintrand
Centre de Recherches sur les Macromolécules Végétales, Grenoble, France
Rachel Auzely-Velty
Centre de Recherches sur les Macromolécules Végétales, Grenoble, France
Catherine Picart
Grenoble Institute of Technology and CNRS, Grenoble, France
Paper No:
SBC2012-80206, pp. 407-408; 2 pages
Published Online:
July 19, 2013
Citation
Boudou, T, Kharkar, P, Jing, J, Guillot, R, Pignot-Paintrand, I, Auzely-Velty, R, & Picart, C. "Polyelectrolyte Multilayer Nanoshells With Hydrophobic Nanodomains for Delivery of Paclitaxel." Proceedings of the ASME 2012 Summer Bioengineering Conference. ASME 2012 Summer Bioengineering Conference, Parts A and B. Fajardo, Puerto Rico, USA. June 20–23, 2012. pp. 407-408. ASME. https://doi.org/10.1115/SBC2012-80206
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