Cryo-preservation of precious cells like stem cells and oocytes requires high success rate because they are hard to obtain and limited in number. Simulation of the freezing process could: (1)Serve as an inexpensive replacement for vivo experiments (when collecting complete experiment data is too costly or time consuming); (2) Help optimize the cyro-preservation protocols. (3) Help to confirm or disprove intracellular ice formation theories. Most of the published models simulating the water loss and intracellular ice formation have treated the cell as a uniform volume with evenly distributed water content. Albeit being a well-founded premise given consideration to the minuteness size of the cell, there are several issues it cannot properly address.
- Bioengineering Division
A Two/Three Dimension Model of Intracellular Ice Formation in Cryo-Preservation
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Li, W, Zhang, A, & Xu, LX. "A Two/Three Dimension Model of Intracellular Ice Formation in Cryo-Preservation." Proceedings of the ASME 2011 Summer Bioengineering Conference. ASME 2011 Summer Bioengineering Conference, Parts A and B. Farmington, Pennsylvania, USA. June 22–25, 2011. pp. 1073-1074. ASME. https://doi.org/10.1115/SBC2011-54022
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