Wall shear stress induced by blood flow affects morphology and physiology of endothelial cells. The specific mechanisms alternating shapes and functions have not been identified in endothelial cells, because of the lack of a detailed description of the flow near the cell surface. To clarify the mechanism, an analysis of the flow on subcellular scale is required. We therefore developed velocimetry using expanded cell model (Fukushima et al. 1997). The method, however, needs some improvements in order to discuss wall shear stress distribution on subcellular scale. In this paper we deal with determination of three-dimensional velocity field and wall shear stress distribution and verify the reliability by comparing experimental results with theoretical solution.