Bio-degradable Poly (l-lactic acid) (PLLA) scaffolds synthesized using thermally induced phase separation (TIPS) method was used to load cryo-preserved human adipose derived stem cells (hASCs). To make the scaffolds, PLLA-Dioxane solutions were formed by dissolving PLLA in 1,4-Dioxane with three different compositions (wt/vol). These PLLA-Dioxane solutions, frozen in three different cooling rates were lyophilized at 0.037bar and −70°C for 48hrs resulting in porous PLLA scaffolds. Based on the porosity, pore size and compressive strength, a suitable scaffold was chosen to investigate its bio-compatibility and osteo-inductive potential.
Osteo-Induction of Human Adipose Derived Stem Cells Cultured on Poly (L-Lactic Acid) Scaffolds Prepared by Thermally Induced Phase Separation Method
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Chinnasami, H, & Devireddy, R. "Osteo-Induction of Human Adipose Derived Stem Cells Cultured on Poly (L-Lactic Acid) Scaffolds Prepared by Thermally Induced Phase Separation Method." Proceedings of the ASME 2015 International Mechanical Engineering Congress and Exposition. Volume 3: Biomedical and Biotechnology Engineering. Houston, Texas, USA. November 13–19, 2015. V003T03A011. ASME. https://doi.org/10.1115/IMECE2015-51906
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