Mixing has become a challenge in micro-fluidic systems because of the low Reynolds number in micro-channels. The method which is implemented in this paper is to use freely-swimming bacteria to enhance the mixing process. Accordingly, the Serratia marcescens bacteria were used for this matter. The mixing performance of the system is quantified by measuring the diffusion rate of Rhodamine B in a particular section of a channel connected to a chamber with varying Rhodamine B concentration. The concentration of Rhodamine B was measured using the Laser Induced Fluorescence (LIF) technique. The channel is in the form of a pipe and is closed on the extending side. In this paper, it is demonstrated that the corresponding diffusion coefficient can be augmented by bacterial participation and that this augmentation can be continued for several hours, depending on the environmental conditions. Additionally, it is shown that the mixing process reacts in response to modifications to the chemical environment of the system, which in turn affect the metabolic activity of the bacteria. Also, a 30 mM glucose buffer was used to show the impact of food on the performance of the bacterial system. It is thus shown that the existence of glucose increases the mixing ability of bacteria.
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ASME 2009 International Mechanical Engineering Congress and Exposition
November 13–19, 2009
Lake Buena Vista, Florida, USA
Conference Sponsors:
- ASME
ISBN:
978-0-7918-4385-7
PROCEEDINGS PAPER
Use of the Freely-Swimming, Serratia Marcescens Bacteria to Enhance Mixing in Microfluidic Systems
Mahyar Peysepar,
Mahyar Peysepar
Sharif University of Technology, Tehran, Iran
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Mohammad Behshad Shafii,
Mohammad Behshad Shafii
Sharif University of Technology, Tehran, Iran
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Ramin Rasoulian,
Ramin Rasoulian
Sharif University of Technology, Tehran, Iran
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Hosein Jamalifar,
Hosein Jamalifar
University of Tehran, Tehran, Iran
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Mohammad Reza Fazeli
Mohammad Reza Fazeli
University of Tehran, Tehran, Iran
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Mahyar Peysepar
Sharif University of Technology, Tehran, Iran
Mohammad Behshad Shafii
Sharif University of Technology, Tehran, Iran
Ramin Rasoulian
Sharif University of Technology, Tehran, Iran
Hosein Jamalifar
University of Tehran, Tehran, Iran
Mohammad Reza Fazeli
University of Tehran, Tehran, Iran
Paper No:
IMECE2009-11469, pp. 463-467; 5 pages
Published Online:
July 8, 2010
Citation
Peysepar, M, Shafii, MB, Rasoulian, R, Jamalifar, H, & Fazeli, MR. "Use of the Freely-Swimming, Serratia Marcescens Bacteria to Enhance Mixing in Microfluidic Systems." Proceedings of the ASME 2009 International Mechanical Engineering Congress and Exposition. Volume 12: Micro and Nano Systems, Parts A and B. Lake Buena Vista, Florida, USA. November 13–19, 2009. pp. 463-467. ASME. https://doi.org/10.1115/IMECE2009-11469
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