The separation of cells from a complex sample by immunomagnetic capture has become a standard technique in the last decade and has also obtained increased attention for microfluidic applications. We present a model that incorporates binding kinetics for the formation of cell-bead complexes, which can easily be integrated into a computational fluid dynamics (CFD) code. The model relies on the three equation types: Navier-Stokes equations governing the fluid dynamics, convection-diffusion equations for non-magnetic cells and a Nernst-Planck type equation governing the temporal evolution of cell-bead complex concentrations. The latter two equations are augmented by appropriate ‘reaction’ terms governing the binding kinetics which is formulated as a population rate balance between creation and annihilation of cell-bead complexes. First, the simulation results show, that by means of the developed approach appropriate parameter sets can be identified which allow for a continuous separation of tagged cells (cell/bead complexes) from non-magnetic particles such as non-target cells entering with the target cells. Moreover tagged cells can be, to a certain extend, separated from unbound beads. Second, the computed concentrations at the outlet show a drastic drop for higher cell/bead complexes beyond a certain number of beads per cell. We show that a critical number of beads per cells exists where the binding is considerably reduced or the reaction cascade ceases completely. This occurs when cell/bead complex have a similar magnetic mobility as the free magnetic beads. The presented CFD model has been applied to the simulation of a generic continuous cell separation system showing that the method facilitates the design of magnetophoretic systems.
- Nanotechnology Institute
Modelling Immunomagnetic Cell Capture in CFD
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Baier, T, Mohanty, S, Drese, KS, Rampf, F, Kim, J, & Scho¨nfeld, F. "Modelling Immunomagnetic Cell Capture in CFD." Proceedings of the ASME 2008 6th International Conference on Nanochannels, Microchannels, and Minichannels. ASME 2008 6th International Conference on Nanochannels, Microchannels, and Minichannels. Darmstadt, Germany. June 23–25, 2008. pp. 1545-1555. ASME. https://doi.org/10.1115/ICNMM2008-62176
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